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Bio Basic Canada pbluescript ks + cloning vector
Pbluescript Ks + Cloning Vector, supplied by Bio Basic Canada, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/pbluescript ks + cloning vector/product/Bio Basic Canada
Average 90 stars, based on 1 article reviews
pbluescript ks + cloning vector - by Bioz Stars, 2026-05
90/100 stars

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cyp1a1_mutA and cyp1a1_mutB clonality assessment. Upper and lower panels show the sequencing results obtained from 16 colonies following cloning of the cyp1a1 gene from cyp1a1_mutA and cyp1a1_mutB, respectively, into <t>pBluescript</t> KS+. The 20 nucleotide sequences targeted by the crRNA is highlighted in grey, PAM sequence is highlighted in black, inserted sequences are highlighted in pink. All sequenced plasmids showed the expected genetic mutation in the cyp1a1 region targeted by the crRNA
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cyp1a1_mutA and cyp1a1_mutB clonality assessment. Upper and lower panels show the sequencing results obtained from 16 colonies following cloning of the cyp1a1 gene from cyp1a1_mutA and cyp1a1_mutB, respectively, into <t>pBluescript</t> KS+. The 20 nucleotide sequences targeted by the crRNA is highlighted in grey, PAM sequence is highlighted in black, inserted sequences are highlighted in pink. All sequenced plasmids showed the expected genetic mutation in the cyp1a1 region targeted by the crRNA
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https://www.bioz.com/result/pbluescript ks( ) apr; high-copy-no. cloning vector/product/Agilent technologies
Average 90 stars, based on 1 article reviews
pbluescript ks( ) apr; high-copy-no. cloning vector - by Bioz Stars, 2026-05
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cyp1a1_mutA and cyp1a1_mutB clonality assessment. Upper and lower panels show the sequencing results obtained from 16 colonies following cloning of the cyp1a1 gene from cyp1a1_mutA and cyp1a1_mutB, respectively, into pBluescript KS+. The 20 nucleotide sequences targeted by the crRNA is highlighted in grey, PAM sequence is highlighted in black, inserted sequences are highlighted in pink. All sequenced plasmids showed the expected genetic mutation in the cyp1a1 region targeted by the crRNA

Journal: Cell & Bioscience

Article Title: A ribonucleoprotein transfection strategy for CRISPR/Cas9‐mediated gene editing and single cell cloning in rainbow trout cells

doi: 10.1186/s13578-021-00618-0

Figure Lengend Snippet: cyp1a1_mutA and cyp1a1_mutB clonality assessment. Upper and lower panels show the sequencing results obtained from 16 colonies following cloning of the cyp1a1 gene from cyp1a1_mutA and cyp1a1_mutB, respectively, into pBluescript KS+. The 20 nucleotide sequences targeted by the crRNA is highlighted in grey, PAM sequence is highlighted in black, inserted sequences are highlighted in pink. All sequenced plasmids showed the expected genetic mutation in the cyp1a1 region targeted by the crRNA

Article Snippet: The purified cyp1a1 PCR product from each mutant cell line and the cloning vector pBluescript KS + were digested with SmaI (New England Biolabs, Ipswich, MA, USA).

Techniques: Sequencing, Clone Assay, Mutagenesis